Chemical structures of the TU-C and TU-Cred products derived from E. coli tRNA
نویسندگان
چکیده
منابع مشابه
The elongation factor EF-Tu from E. coli binds to the upstream activator region of the tRNA-tufB operon.
The polypeptide chain elongation factor EF-Tu of Escherichia coli is encoded by two genes, tufA and tufB, located in two different operons. Experiments in which either tufA or tufB was inactivated demonstrated that expression of the tRNA-tufB operon is dependent on a functioning tufA and thus on EF-Tu (1, to be published). In order to study a possible role of EF-Tu as trans-activator of the tRN...
متن کاملProperties of Escherichia coli EF-Tu mutants designed for fluorescence resonance energy transfer from tRNA molecules.
Here we describe the design, preparation and characterization of 10 EF-Tu mutants of potential utility for the study of Escherichia coli elongation factor Tu (EF-Tu) interaction with tRNA by a fluorescence resonance energy transfer assay. Each mutant contains a single cysteine residue at positions in EF-Tu that are proximal to tRNA sites within the aminoacyl-tRNA.EF-Tu.GTP ternary complex that ...
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This work presents an algorithm for injectively parameterizing surfaces into spherical target domains called spherical orbifolds. Spherical orbifolds are cone surfaces that are generated from symmetry groups of the sphere. The surface is mapped the spherical orbifold via an extension of Tutte’s embedding. This embedding is proven to be bijective under mild additional assumptions, which hold in ...
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متن کاملThe tRNA specificity of Thermus thermophilus EF-Tu.
By introducing a GAC anticodon, 21 different Escherichia coli tRNAs were misacylated with either phenylalanine or valine and assayed for their affinity to Thermus thermophilus elongation factor Tu (EF-Tu)*GTP by using a ribonuclease protection assay. The presence of a common esterified amino acid permits the thermodynamic contribution of each tRNA body to the overall affinity to be evaluated. T...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80769-5